Anti Fibronectin (human)
Mouse monoclonal antibody
Cat.No. HYB 051-05
Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL
Concentration: 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15mM sodium azide
Storage: In the dark at 4-8ºC
Fibronectin is an adhesive glycoprotein with a molecular mass of 440 kDa. It is believed to be important for the formation of a provisional matrix that promotes cell adhesion and migration during wound healing. Its age-dependent increase in plasma and tissues may be accompanied in pathological states, especially in tumor growth, by its proteolytic breakdown by a number of neutral proteases. It has also shown that several of its proteolytic breakdown products exhibit unexpected and mostly harmful biological activities.
Fibronectin isolated from human plasma.
HYB 051-05 has specificity for human fibronectin
Not determined
HYB 051-05 reacts strongly with fibronectin. Strong reaction is seen in ELISA with fibronectin directly coated onto the microtiter plate.
In Western blot after SDS-PAGE, HYB 051-05 reacts with fibronectin in reduced as well as in nonreduced form.
| Method | Usability | Dilution guideline | References |
| ELISA | Yes | 1:5000 | |
| Immunoblotting | Yes | ||
| Immunohistochemistry | ND |
1. Underwood PA, Dalton BA, Steele JG, Bennett FA, Strike P (1992) Anti-fibronectin antibodies that modify heparin binding and cell adhesion: evidence for a new cell binding site in the heparin binding region. J Cell Sci 102:833-845.
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HYB 051-05.pdf (22.41 kB) Product specification
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Antibody MSDS
April 23 2008
